# Tibco TCP-BW5 : TIBCO ActiveMatrix BusinessWorks 5 Exam

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Exam Number : TCP-BW5
Exam Name : TIBCO ActiveMatrix BusinessWorks 5
Vendor Name : Tibco
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### Longitudinal in vivo imaging of acute neuropathology in a monkey model of Ebola virus an infection | TCP-BW5 PDF Dumps and Cheatsheet

Animal care and use

All animal experiments had been carried out in a BSL-4 laboratory, were authorised by way of the NIAID Division of clinical research Animal Care and Use Committee, and have been performed in an AAALAC foreign accepted facility in line with significant NIH guidelines and the Animal Welfare Act and laws.

For the MRI cohort (community A), 10 male Indian beginning rhesus macaques (Macaca mulatta, age range: 23–sixty six months, average age: 42.3 ± 19.9 months; normal weight: 5.2 ± 1.8 kg) have been used. All subjects have been inoculated with 1000 plaque forming contraptions of EBOV, Makona isolate (EBOV-Makona) by the use of intramuscular injection. Two baseline brain MR scans were carried out on every animal, and two or three MR scans have been carried out submit-an infection: on days 2 and 4 (n = three), days three and 6 (n = three), or days 1, 5, and 6/7 (n = four).

For the FDG PET/CT cohort (neighborhood B), 15 male Indian beginning rhesus macaques (Macaca mulatta, age latitude: 31–91 months, commonplace age: forty eight ± 18.2 months, normal weight: 5.6 ± 2.1 kg) had been used. All subjects were inoculated with 1000 plaque forming devices of EBOV-Makona by way of intramuscular injection. group B was divided right into a serial sacrifice subgroup (n = 7), by which animals were euthanized at day 2, day 5, and Terminal (days 7 or 8) publish-exposure (n = 8). The terminal community Topics have been allowed to development except meeting medical terminal endpoints68. All animals bought two baseline FDG PET/CT scans, however the variety of comply with-up scans distinct through subgroup task. Serial sacrifice animals euthanized at day 2 received one publish-inoculation PET/CT scan instantly in advance of euthanasia. Serial sacrifice animals that had been euthanized at day 5 obtained two publish-inoculation FDG PET/CT scans, at days 2 and 5, automatically previous to euthanasia. Terminal animals had been scanned either three instances (at days 1/2, 5, and seven/8) or four times just before assembly endpoint criteria (at days 1, 4, 6, and seven/eight).

a 3rd neighborhood of male rhesus macaques (n = 2, Macaca mulatta, age range: 24-36 months, commonplace age: 30 ± 8.5 months, typical weight: four.5 ± 0.5 kg), also contaminated with EBOV-Makona, had been evaluated at baseline and on day 6 after inoculation the use of structural imaging together with Proton-density/T2 (double-echo), pre and publish distinction second T2 aptitude images, as well as T2*-weighted imaging. The scans were evaluated qualitatively by way of a neuroradiologist (DAH) with 18 years of adventure.

Biomarker sampling

For all animals in the experiment, complete blood samples had been bought as a minimum twice just before EBOV publicity and subsequently on each post-an infection scan day except euthanasia. comprehensive blood counts with differential (CBC/diff) were carried out at different time elements in all animals to examine disease progression. This blanketed assessment of white blood mobilephone counts in addition to counts and percentages of monocytes, lymphocytes, reticulocytes, neutrophils, and platelets. CBC/diff changed into decided from blood samples amassed in ethylenediaminetetraacetic acid (EDTA)-covered blood tubes and analyzed the use of a Sysmex XT2000V™ (Sysmex america, Mundelein, IL). For group A best, CSF samples had been also received via cisternal puncture at baseline and due to this fact on days four, 5, 6, or 7 submit infection.

Plasma and CSF qPCR assays had been determined using the BEI materials crucial Reagents software (CRP) EZ1 quantitative reverse transcription PCR (qRT-PCR) package. Serum and CSF cytokines had been assessed using a Milliplex NHP primate package (Millipore) that has been validated to pass react with rhesus macaques. Viral RNA from each plasma and CSF have been analyzed with the identical qPCR assay, which removed the inter-assay variability as a concern. In quick, viral RNA become extracted from 70 µl of pattern that became inactivated by means of Trizol LS, and introduced to 280 µl of buffer AVL (Qiagen, Gemantown, MD Cat No. 19073). Samples were then extracted the use of the QIAamp Viral RNA Mini package (Qiagen, Germantown, MD Cat No 52904) in line with the manufacturer’s guidelines, eluted in 70 µl of buffer AVE (Qiagen), aliquoted, and frozen. five microliters of pattern were assayed in reproduction reactions using the Ebola Zaire goal 1 LightCycler/speedy master mix (Cat. No. BEI CRP PCR-EBZ-1R-k) and compared to a standard curve of serial tenfold dilutions (Cat No. BEI CRP Ebola Zaire target 1 custom Conc. superb manage 1E + 9 copies/rxn). The reduce limit of detection of our assay is 100 viral RNA copies.

For viral titration in distinctive brain regions, tissues samples have been first collected, snap frozen at -80oC and a ten% homogenate (w/v) turned into prepared in PBS the use of a Bead Ruptor Elite (Omni-inc., Georgia, united states of america). The tissue samples have been then serially diluted and added to just about confluent VeroE6 cells (BEI resources, a division of american classification culture collection (ATCC)) grown in multiwell plates. For the subsequent hour, the virus adhered to and contaminated the cells (37 °C/5% CO2). once the cells were infected, semi-solid 1.25% Avicel (FMC Biopolymer) diluted in EMEM was brought to the corresponding wells, incubated for five days (37 °C/5% CO2) and eventually aspirated. At that element, the cells were mounted and stained the use of impartial buffered formalin (NBF, 0.four% crystal violet) for 30 min at room temperature. at the conclusion of the assay, the plates had been washed with water and the plaques had been counted.

Imaging procedures

All MRI scans, together with these of community A, and 9 of the fifteen animals in group B, have been performed using a 3 Tesla Philips Achieva MRI (R3, v3.2.1.0) (Philips Healthcare, Cleveland, OH, u . s .) with an 8-channel pediatric experience head-backbone coil. prior to the scan, subjects had been anesthetized with ketamine (15 mg/kg, IM), then intubated, immobilized using isoflurane (2‒2.5%) inhalation, located supine on the scanner mattress and monitored all over anesthesia. For consistency of positioning, and to avoid action, the animals’ heads had been immobilized internal the pediatric feel head-backbone coil the use of smooth pads. particular care was taken to make certain head positioning turned into constant between animals, in addition to between the diverse scanning sessions of the same animals. This stability become established the usage of the scout views previous to initiation of the scanning.

For the animals in community A, T1 maps have been generated using a dual-flip-angle method28. For this, two FFE photographs (60 slices, box of view (FOV): one hundred ten ×one hundred ten x 60 mm, slice thickness: 1.0 mm, matrix: 220 × 220) had been got in an axial orientation using a twin-flip attitude SPGR protocol (TR/TE/FA1/FA2 = 34 ms/three.four ms/5o/32o), each pre- and post-contrast. A phantom turned into positioned in the FOV to serve as a normalization aspect in T1 estimation of publish-contrast scans. For this a part of the study, Gadovist® (Gadobuterol, Bayer AG, Germany) become administered intravenously at 0.1 ml/kg. The timing of distinction administration and initiation of imaging changed into stored strictly constant throughout all animals and all scans of the identical animals.

Accuracy and precision of T1 relaxation times’ measures from the dual-flip-angle system had been assessed the usage of gel phantoms (Leeds look at various Object, North Yorkshire, UK) and a healthy NHP. First, an inversion healing approach used to scan the phantoms offered measures inside the estimated accuracy reported by way of the brand for each and every phantom and became used as a floor actuality. The phantoms were scanned at distinct instances, days, and orientations and the coefficient of variance with recognize to the range ordinary for white and gray depend was measured. at last, uninfected NHPs were scanned distinctive times inside 1 day and the coefficient of adaptation of T1 values within ten selected areas of activity within the mind was measured.

For the animals in neighborhood B, FDG PET/CT scans had been performed with a Philips Gemini PET/CT scanner (Philips Healthcare, Cleveland, OH, united states). earlier than the scan, Topics had been anesthetized with ketamine (15 mg/kg, IM), then intubated, immobilized the use of isoflurane (2‒2.5%) inhalation, positioned supine on the scanner mattress and monitored all through anesthesia. CT images had been obtained in helical scan mode with the following parameter settings: 140 kVp, 250 mAs/slice, three mm thickness, 1.5 mm increment, 0.688 mm pitch, collimation sixteen×0.seventy five and 0.5 s rotation. Two CT photos were reconstructed from the uncooked records. An initial CT graphic became reconstructed right into a 600-mm diameter container-of-view (FOV), leading to a pixel dimension of 1.17 mm, and slice spacing of three mm. This CT picture was used to relevant the PET photos for photon attenuation. The uncooked CT information were reconstructed a second time right into a diagnostic fine CT photograph via decreasing the FOV size to 250 mm, which resulted in a pixel dimension of 0.352 mm, and a slice spacing of 1.0 mm. All CT images have been reconstructed into a 512 × 512 matrix using the usual “B” filter. No distinction changed into given, and the subjects have been freely respiration all over the scan. The diagnostic great scans were reviewed through an experienced neuroradiologist (DAH) to rule out any structural mind abnormalities. Following the CT scan, a static PET emission scan covering the top became performed (FDG dose = 2.sixteen ± 0.15 mCi). The uptake duration become 50 min. The scanner offers an axial FOV size of 180 mm for a single mattress position. The scanning time became 5 min for one field of view. PET records had been acquired in record mode and reconstructed the usage of Philips’ iterative, highest-chance reconstruction algorithm (3D-RAMLA) (prolonged Brilliance Workspace (EBW) PET/CT software suite v1.30 29-Nov-2006) into photos with a 128 × 128 matrix size and a pair of mm huge cubic voxels. To be certain quantitative accuracy, all reconstructed PET pictures had been corrected for radioactive decay all the way through the scan, uniformity, random coincidences, attenuation and scattering. each PET and CT photographs were then despatched for radiologic interpretation and evaluation. For 9 out of 15 animals in community B, structural brain MR imaging turned into carried out the usage of 3D MPRAGE (TE: 4.715 ms, TR: 9.seventy five ms, matrix: ninety six × 96 x sixty eight mm, slice thickness: 0.5 mm).

For the animals in neighborhood C, structural imaging become performed together with here sequences: Proton density/T2 weighted imaging the usage of a multi-echo sequence (TE = 20, 40, 60, and eighty msec; in-plane decision = 0.forty three × 0.forty three mm; slice thickness = 2 mm, TR = 3300 ms, flip attitude = ninety°, fat suppression the usage of SPIR, NSA = 1, and FOV = 96 × 96 x 60 mm); Susceptibility-weighted imaging the usage of a principle of echo shifting/R2*(PRES R2*; 3D, FFE, in-plane decision = 0.5 × 0.5 mm, slice thickness = 0.5 mm using over-contiguous slices; TR/TE = 34/42 ms, NSA = 1, flip attitude = 5o, and FOV = 96 × 96 x 60 mm); Pre and post distinction T2 aptitude imaging (turbo spin echo (TSE) sequence, in-plane resolution = 0.5 × 0.5 mm, slice thickness = 1 mm, TR = 10,000 ms, TE = a hundred ms, NSA = 1, fat suppression the usage of SPIR, TSE element = 9, and FOV = one hundred twenty five ×125 mm). In these animals, Magnevist® (Gadopentetate dimeglumine, Schering, Berlin, Germany) became administered intravenously at 0.2 ml/kg.

photograph evaluation

analysis of imaging data changed into performed using MIM utility, version 7.0.four (Cleveland, Ohio). For group A, the D99 brain atlas turned into warped to fit a T1-weighted scan that became co-registered to the T1 maps69. The accuracy of registration of the D99 atlas was Checked for all animals earlier than performing any further analysis. Volumes of pastime (VOIs) together with the whole brain as neatly as the frontal cortex, centrum semiovale, caudate, putamen, thalamus, hippocampus, midbrain, pons, medulla oblongata, and cerebellar white/grey matter were delineated on the D99 atlas. The VOIs have been consequently gotten smaller circumferentially (latitude of 0.four–0.6 mm in all instructions) to prevent partial averaging with CSF containing buildings. The VOIs have been then overlaid on co-registered pre- and submit-contrast T1 maps. The phantom T1 values had been used to normalize the publish-contrast T1 values earlier than calculating the percent alternate in T1 after contrast administration. the usage of these data, we received T1 pre-contrast values for each subregion demarcated through the D99 atlas. We additionally calculated percentage submit-distinction T1-shortening (%T1-shortening) for every location through subtracting the publish-T1 value from the pre-T1 cost and dividing the product by way of the pre-T1 price ((Pre-submit)/Pre)*100%).

For group B, the CT, MRI, and PET scans for a given scanning session had been co-registered to 1 yet another. The identical VOIs as above have been automatically positioned on the MRI mind through the use of the D99 template/atlas and then utilized to the co-registered PET scan69. Contracting the VOIs circumferentially, as described above, helped lessen the potential for spill in/spill out (partial quantity effect). The appropriate vicinity of the VOIs following co-registration changed into checked prior to performing the analysis. VOIs protected the total brain as well as the regional VOIs (frontal cortex, centrum semiovale, caudate, putamen, thalamus, hippocampus, medulla, midbrain, pons and cerebellar white/gray rely). For subjects with out MRI scans, an age- and weight-matched animal’s brain MRI become used to more precisely vicinity the VOIs onto the PET scan.

once the VOIs had been placed, the corresponding commonplace Standardized Uptake Values (SUVmean) had been calculated as follows (1):

$$\rmSUVmean=\frac\rmRegional\; \rmradioactivity\; \rmconcentration\rmAdministered\; \rmradiotracer\; \rmdose/\rmbody\; \rmweight$$

(1)

We then calculated relative SUV values through normalizing regional FDG uptake to entire mind FDG uptake, as follows (2):

$$\rmRelativeSUV=\frac\rmRegional\; \rmSUVmean\rmentire\; \rmbrain\; \rmSUVmean$$

(2)

Histology

Quantification of the immunofluorescent staining became performed the use of FIJI photograph processing package, according to ImageJ, edition 1.eight.0 (NIH, Bethesda, MD). The RGB bitmap photographs were first converted to eight-bit grayscale and the brink adjusted to consist of only cells of hobby and eliminate the background. This changed into adopted by using calculating the fluorescence intensity within carefully selected areas of hobby (ROIs). on the grounds that the stained regions have been a little bit heterogeneous because of moderate variability within the orientation of the slides, about 70–one hundred small fields of view have been carefully chosen to supply consistency for quantifying staining throughout all animals. The facts for all stains is expressed as p.c fluorescence depth relative to the enviornment of the ROI. We also carried out a visible evaluation of a variety of neuronal mobile our bodies to check for the percentage of VP40-fine cells compared to VP40-negative cells and to investigate no matter if some neurons confirmed apoptotic markers without being VP40 advantageous. anyway measuring Iba1 fluorescent depth, we looked for early refined signals of activation reminiscent of increasing size of microglial cell body, and shortening/thickening of microglial strategies. ultimately, for each and every animal, we randomly selected six ROIs of the equal measurement from distinctive sections of the brainstem slides. We then manually delineated 12 mobile our bodies in each ROI, the usage of image J free fingers alternative tool, for a total of seventy two cells per animal. The average size of the microglial cell bodies changed into then estimated and said in area (pixels).

statistics

with a purpose to reduce the effect of sepsis and multi-organ failure on our consequences, we eradicated imaging records received in the late stage of the sickness (days 7 and 8) for all analyses. Statistical evaluation turned into carried out the usage of S-plus application, edition eight.2 (TIBCO utility Inc, Palo Alto, California). The lme4 package70 (Code = lme), a developed-in function in S-plus 8.2, changed into used.

For community A, linear mixed fashions (LMM) have been used to evaluate the impact of time put up-infection (unbiased variable) on T1 pre-contrast values and %T1-shortening (stylish or outcomes variables) in each and every VOI. Linear blended-mannequin analysis can determine a significant universal impact of time on T1 values and %T1-shortening and measure the magnitude and importance of the connection between specific time aspects and alterations in these values. records from sequential days had been grouped (Baseline, Day 1, Day 2/three, Day four/5, Day 6) to boost the sample measurement of every time factor. Random intercepts were used. similar LMMs had been used to consider the impact of time put up an infection (independent variable) on CSF cytokine tiers (dependent variables). value became determined as p-value <0.01 to avoid category I mistakes. magnitude traits had been assigned for p-values between 0.01 and zero.05.

extra LMMs have been created to look at various for correlations between MRI-derived measures (T1 and %T1 shortening as based variables) and CSF cytokines, CSF viral load, and plasma viral load (unbiased variables). Biomarker values had been transformed by using taking the log10 values in instances the place it produced a greater commonplace distribution of data. magnitude turned into decided as p-price < 0.01 to stay away from type I errors.

For neighborhood B, analysis was carried out with both absolute and relative mean SUVs. Relative SUVs have been calculated via normalizing the SUVs of regional VOIs to the SUV of the total mind to detect regional changes in the surroundings of global hypo- or hypermetabolism. in order to look at various for changes in FDG uptake over the direction of ailment, LMMs were built with SUV or relative SUV as the stylish variable and time as the impartial variable to examine even if there became a big normal impact of time on SUV and to measure the magnitude and significance of the relationship between particular time elements and changes in FDG uptake. For this analysis, facts from sequential days were grouped (Baseline, Days 1/2, Days four/5, Day 6) to increase the sample size of each and every time point. an identical LMMs had been used to evaluate the impact of time put up infection (independent variable) on plasma cytokine stages (stylish variables). value turned into determined as p-cost < 0.01 to steer clear of class I error. importance developments were assigned for p-values between 0.01 and 0.05. Linear combined models additionally assessed the relationships between FDG uptake (dependent variables) and other markers of disorder including viral load, plasma cytokine tiers, and measures from complete blood counts (unbiased variables). Biomarker values were changed by taking the log10 values in situations the place it produced a greater ordinary distribution of facts. magnitude was decided as p-price < 0.01 to avoid class I blunders.

Reporting summary

extra counsel on research design is accessible within the Nature analysis Reporting summary linked to this article.

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